Review





Similar Products

bfa monensin mixture  (Multi Sciences (Lianke) Biotech Co Ltd)
95
Multi Sciences (Lianke) Biotech Co Ltd bfa monensin mixture
Bfa Monensin Mixture, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/BFA-Monensin+Mixture/pmc13189296-334-9-14
Average 95 stars, based on 1 article reviews
bfa monensin mixture - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
brefeldin a bfa  (MedChemExpress)
98
MedChemExpress brefeldin a bfa
Brefeldin A Bfa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/Brefeldin+A/pmc13469471-48-0-3
Average 98 stars, based on 1 article reviews
brefeldin a bfa - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier
brefeldin a  (InvivoGen)
95
InvivoGen brefeldin a
Brefeldin A, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/Brefeldin+A/custom%40inh-bfa-2%4010%2E64898%2F2026%2E07%2E09%2E737484
Average 95 stars, based on 1 article reviews
brefeldin a - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
bfa  (MedChemExpress)
98
MedChemExpress bfa
Bfa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/Brefeldin+A/pm42332681-78-35-38
Average 98 stars, based on 1 article reviews
bfa - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier
brefeldin a bfa treatment  (Thermo Fisher)
95
Thermo Fisher brefeldin a bfa treatment
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Brefeldin A Bfa Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/Brefeldin+A%2C+natural/bio_rxiv__64898__2026__05__13__724867-140-3-15
Average 95 stars, based on 1 article reviews
brefeldin a bfa treatment - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

Image Search Results


KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

Journal: bioRxiv

Article Title: Degradation of cytokinesis-specific Qa-SNARE KNOLLE is regulated by context-dependent ubiquitination

doi: 10.64898/2026.05.13.724867

Figure Lengend Snippet: KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

Article Snippet: Estradiol induction and brefeldin A (BFA) treatment: 50 μM BFA (50 mM stock in DMSO/Ethanol, Invitrogen) was applied for 30 minutes, followed by 20 μM estradiol (20 mM stock in DMSO, Sigma-aldrich) for 3 hours and 30 minutes in the presence of BFA and subsequently wortmannin and NEM were added as described above.

Techniques: Ubiquitin Proteomics, Comparison, Transgenic Assay, Control, Immunoprecipitation